![HHBS [Hanks' Buffer with 20 mM Hepes]: Essential Solutions for Efficient Cell Culture](/web/image/blog.post/34/author_avatar/HHBS%20%5BHanks'%20Buffer%20with%2020%20mM%20Hepes%5D:%20Essential%20Solutions%20for%20Efficient%20Cell%20Culture?unique=94e2fed)
In the world of cell culture and molecular biology, maintaining cell health and experimental consistency is paramount. One reagent that has become indispensable in labs worldwide is HHBS [Hanks' Balanced Salt Solution with 20 mM Hepes]. Known for its versatility and reliability, HHBS supports a wide range of applications, from cell washing to sample transport.
What is HHBS [Hanks' Buffer with 20 mM Hepes]?
HHBS combines Hank’s Balanced Salt Solution (HBSS) with 20 mM Hepes, a buffering agent that stabilizes pH in cell culture conditions. This ensures cells remain healthy during critical procedures, including:
- Transporting cells or tissue samples
- Washing and diluting cells for counting
- Preparing reagents for experiments
The Hepes component helps maintain physiological pH even in ambient CO₂ conditions, making HHBS ideal for sensitive cells.
Key Features of HHBS
- Stable pH environment: Maintains optimal conditions for cells during manipulation
- Versatile applications: Suitable for washing, transport, dilution, and reagent preparation
- Compatible with multiple cell types: Ideal for primary cells, stem cells, and immortalized cell lines
- Ready-to-use format: Comes in convenient 100 mL volumes for laboratory efficiency
Why HHBS is Critical in Cell Culture
HHBS is more than a simple buffer it’s a foundation for experimental reliability. In cell culture experiments, cells are highly sensitive to changes in pH and osmolarity. Using HHBS ensures:
- Minimal stress on cells during handling
- Accurate cell counting and viability measurements
- Consistent experimental results across replicates
Whether you are conducting immunology assays, stem cell research, or tissue engineering, HHBS is a trusted tool in the scientist’s toolkit.
Applications of HHBS in the Laboratory
Cell Washing: Remove residual media and prepare cells for downstream assays.
Sample Transport: Preserve cells and tissues during transfer between laboratories or storage conditions.
Dilution of Cells: Ensure accurate cell counting with minimal stress on samples.
Reagent Preparation: Use HHBS as a stable buffer base for experimental reagents.
Why Choose HHBS from Trusted Suppliers
At Thymistem, we provide high-quality HHBS with 20 mM Hepes, ensuring reproducibility and reliability in your experiments. Our products are manufactured under strict quality control to meet research-grade standards, making them ideal for both academic and industrial laboratories.