💡 Learn how to detect and quantify nucleic acids in real-time
What you’ll learn:
Principles of fluorescence detection (SYBR Green, TaqMan)
qPCR vs conventional PCR
Setting up standard curves and quantification
Ct/Cq values interpretation
Avoiding false positives/negatives
Efficiency, R², and melt curve analysis
Troubleshooting low amplification or non-specificity
Who is it for?
🔹 Clinical labs, diagnostic labs, research teams using RT-PCR for gene expression, pathogens, SNPs, etc.
🕒 Duration: 3 hours
📜 Certificate: Yes – completion & skills certificate
🧪 Includes: downloadable protocols + virtual qPCR simulation
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